Fluoromount-G熒光封片劑（不含不含DAPI）

產(chǎn)品描述：Fluoromount-G?封片劑是一種水溶性化合物，該熒光封片劑水溶性好，可在實(shí)驗(yàn)最后封片時(shí)使用。由于Fluoromount-G?封片劑是水溶性的，因此可將載玻片浸入PBS溶液中，直到蓋玻片松開，從而移開蓋玻片。使用該熒光封片劑可以減少熒光淬滅，便于更好的分析實(shí)驗(yàn)結(jié)果。
產(chǎn)品組分：PBS,磷酸鹽緩沖液，甘油，0.21%疊氮鈉
體積：25ml
保存：22-25℃
應(yīng)用：免疫組化-冰凍切片
      免疫組化-石蠟切片
      原位雜交
      細(xì)胞凋亡
產(chǎn)品訂購：

封片是將組織切片封固保存于載玻片與蓋玻片之間,使之不與空氣發(fā)生接觸，防止其氧化、褪色,利于鏡檢觀察及保存。不可逆的光漂白會(huì)造成熒光信號(hào)的損失,從而導(dǎo)致靈敏度降低,尤其在靶標(biāo)分子豐度較低、激發(fā)光較強(qiáng)或激發(fā)持續(xù)時(shí)間長(zhǎng)時(shí)，表現(xiàn)得非常明顯。SouthernBiotech公司專i ]開發(fā)出的Fluorescent-G系列是抗熒光淬滅封片劑為您解決熒光信號(hào)淬滅問題。該系列熒光封片劑水溶性好,包括偶聯(lián)DAPI熒光封片劑和不帶偶聯(lián)的熒光封片劑,可在實(shí)驗(yàn)最后后封片時(shí)使用。使用該偶聯(lián)DAPI熒光封片劑不僅可以減少熒光淬滅,還可同時(shí)將細(xì)胞核染成藍(lán)色,以將封片和復(fù)染-步完成。便于更好的分析實(shí)驗(yàn)結(jié)果。

點(diǎn)擊查看產(chǎn)品說明書：

操作流程：

1、準(zhǔn)備好染色后的切片或爬片。

2、去除樣本周圍多余的液體,加入1滴(~50ul)封片劑,覆蓋區(qū)域22x22mm。

3、蓋上蓋玻片,去除多余的封片劑。

4、空氣中晾干5min后檢測(cè)。

產(chǎn)品應(yīng)用：

適用于組織切片、細(xì)胞圖片/爬片

實(shí)例

參考文獻(xiàn)：

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