TNF-alpha/TNFSF2 蛋白, Human

TNF-alpha/TNFSF2 蛋白, Human是一種促炎性細(xì)胞因子，同時能夠高效地靶作用于腫瘤細(xì)胞，而對正常細(xì)胞毒性很低。

種屬：Human

表達(dá)系統(tǒng)：E. coli

蛋白編號：P01375 (V77-L233)

基因 ID：7124

中文名：重組人腫瘤壞死因子-α/TNFSF2

同用名：rHuTNF-α/TNFSF2; TNF-alpha; Cachectin; DIF; TNFA; Differentiation-inducing factor

氨基酸序列：

VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

分子量：Approximately 17.3 kDa

生物活性：The ED₅₀ is <30 pg/mL as measured by L-929 mouse fibrosarcoma cells, corresponding to a specific activity of >3.3 × 10⁷ units/mg.

性狀：Lyophilized powder.

組分：Lyophilized after extensive dialysis against PBS.

內(nèi)du素含量：<0.2 EU/μg, determined by LAL method.

復(fù)溶方法：It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O or PBS.

保存條件 & 期限：Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.

描述：Tumor necrosis factor α (TNF-α, also known as cachectin) is a strong pro-inflammatory cytokine playing an important role in the immune system during inflammation, cell proliferation, differentiation and apoptosis. TNF-α is type II transmembrane protein that can be expressed in both membrane-bound and secreted forms. Receptors for TNF-a are part of TNF receptor (TNFR) superfamily, with characteristic intracellular segment, a transmembrane domain, and an extracellular ligand-binding domain. They are type I transmembrane glycoproteins characterized by a cysteinerich motif (up to 40 aminoacids) repeated 2-6 times in the extracellular N-terminal domain^[1]. Tumor necrosis factor-α (TNF-α) is a pleiotropic factor exhibiting antitumor effects, alters endothelial barrier function, reduces tumor interstitial pressure and mediates immune responses. TNF-α is associated with a number of cell signalling events, associated with cell necrosis and apoptosis, and is important for resistance to infection and cancer^[2]

參考文獻(xiàn)

[1]. Zelová H, et al. TNF-α signalling and inflammation: interactions between old acquaintances. Inflamm Res. 2013 Jul;62(7):641-51.

[2]. Ren H, et al. Matrix metalloproteinase-mediation of tumor targeting human recombinant tumor necrosis factor-α fusion protein. Mol Med Rep. 2015 Aug;12(2):2035-42.