Lipofectamine? 2000 轉(zhuǎn)染試劑

Lipofectamine? 2000轉(zhuǎn)染試劑是一種多用途轉(zhuǎn)染試劑，可在各種貼壁和懸浮細(xì)胞系中提供高效轉(zhuǎn)染。研究人員可使用Lipofectamine? 2000試劑進(jìn)行基于siRNA和shRNA的基因敲除實(shí)驗(yàn)及基因表達(dá)研究。Lipofectamine? 2000是Life Technologies提供的用于siRNA和質(zhì)粒DNA共轉(zhuǎn)染的較佳試劑。

采用Lipofectamine? 2000試劑，您可以實(shí)現(xiàn)：

? 在各種細(xì)胞系中具有好的轉(zhuǎn)染效率和高重組蛋白表達(dá)水平 (表1)

? 出眾的siRNA和質(zhì)粒DNA共轉(zhuǎn)染性能

? 在血清存在時(shí)亦具有可靠的轉(zhuǎn)染效率 – 轉(zhuǎn)染后無(wú)需更換培養(yǎng)基

? 適用于高通量應(yīng)用的可靠性能

? 建立穩(wěn)定細(xì)胞系的優(yōu)選

適用于基因表達(dá)和基因沉默的高性能轉(zhuǎn)染試劑

Lipofectamine? 2000試劑適合所有常見細(xì)胞系及許多難以轉(zhuǎn)染的細(xì)胞系，可用于含有或不含血清的培養(yǎng)基。在基因沉默應(yīng)用中，Lipofectamine? 2000試劑的高效轉(zhuǎn)染提供了很高的基因敲除水平，足以獲得令人信服的結(jié)果。Lipofectamine? 2000試劑可實(shí)現(xiàn)siRNA和質(zhì)粒DNA的高效轉(zhuǎn)染，它是共轉(zhuǎn)染的優(yōu)選試劑。Lipofectamine? 2000試劑使用方便 – 只需將其與核酸混合，加入細(xì)胞培養(yǎng)物即可。

適合高通量操作

Lipofectamine? 2000試劑使用簡(jiǎn)單、快速且具有很高的轉(zhuǎn)染效率，適用于瞬時(shí)蛋白表達(dá)或高通量RNAi轉(zhuǎn)染。在上述應(yīng)用中，可輕松建立自動(dòng)化或機(jī)器人系統(tǒng)轉(zhuǎn)染條件。如需了解有關(guān)Lipofectamine? 2000的實(shí)驗(yàn)方案、文獻(xiàn)引證及其它產(chǎn)品信息，可登錄細(xì)胞系數(shù)據(jù)庫(kù)。

Lipofectamine?2000轉(zhuǎn)染方案/質(zhì)粒轉(zhuǎn)染

脂質(zhì)體胺? 2000轉(zhuǎn)染示例

引用和文獻(xiàn)：

Molecular and Biochemical Characterization of Rat epsilon -N-Trimethyllysine Hydroxylase, the First Enzyme of Carnitine Biosynthesis.

• 作者:Vaz F M; Ofman R; Westinga K; Back J W; Wanders R J;

• 期刊:J Biol Chem

• PubMed ID:11431483

A novel ankyrin repeat-containing gene (Kank) located at 9p24 is a growth suppressor of renal cell carcinoma.

• 作者:Sarkar Shubhashish; Roy Badal Chandra; Hatano Naoya; Aoyagi Teiichiro; Gohji Kazuo; Kiyama Ryoiti;

• 期刊:J Biol Chem

• PubMed ID:12133830

Naturally occurring mutations in the largest extracellular loops of ABCA1 can disrupt its direct interaction with apolipoprotein A-I.

• 作者:Fitzgerald ML, Morris AL, Rhee JS, Andersson LP, Mendez AJ, Freeman MW.

• 期刊:J Biol Chem

• PubMed ID:12084722

The identification and characterization of a noncontinuous calmodulin-binding site in noninactivating voltage-dependent KCNQ potassium channels.

• 作者:Yus-Najera Eva; Santana-Castro Irene; Villarroel Alvaro;

• 期刊:J Biol Chem

• PubMed ID:12032157

cAMP-dependent protein kinase regulates desensitization of the capsaicin receptor (VR1) by direct phosphorylation.

• 作者:Bhave Gautam; Zhu Weiguo; Wang Haibin; Brasier D J; Oxford Gerry S; Gereau Robert W 4th;

• 期刊:Neuron

• PubMed ID:12194871

Human apical sodium-dependent bile salt transporter gene (SLC10A2) is regulated by the peroxisome proliferator-activated receptor alpha.

• 作者:Jung Diana; Fried Michael; Kullak-Ublick Gerd A;

• 期刊:J Biol Chem

• PubMed ID:12055195

The activated glucocorticoid receptor modulates presumptive autoregulation of ribosomal protein S6 protein kinase, p70 S6K.

• 作者:Shah O Jameel; Iniguez-Lluhi Jorge A; Romanelli Angela; Kimball Scot R; Jefferson Leonard S;

• 期刊:J Biol Chem

• PubMed ID:11705993

Transcriptional Regulation of Kaposi's Sarcoma-associated Herpesvirus-encoded Oncogene Viral Interferon Regulatory Factor by a Novel Transcriptional Silencer, Tis.

• 作者:Wang Xin-Ping; Zhang Yan-Jin; Deng Jian-Hong; Pan Hong-Yi; Zhou Fu-Chun; Gao Shou-Jiang;

• 期刊:J Biol Chem

• PubMed ID:11821384

A highly effective dominant negative alpha s construct containing mutations that affect distinct functions inhibits multiple Gs-coupled receptor signaling pathways.

• 作者:Berlot Catherine H;

• 期刊:J Biol Chem

• PubMed ID:11927592