Lipofectamine? RNAiMAX轉(zhuǎn)染試劑可在多種細(xì)胞類型中獲得較高的轉(zhuǎn)染效率，適用于siRNA介導(dǎo)的基因抑制實(shí)驗(yàn)。Lipofectamine? RNAiMAX是一種獨(dú)有的RNAi特異性陽(yáng)離子脂質(zhì)體轉(zhuǎn)染試劑，適合將siRNA和miRNA輸送至所有細(xì)胞類型中。

采用Lipofectamine? RNAiMAX轉(zhuǎn)染試劑，您可以實(shí)現(xiàn)：
? 超高的轉(zhuǎn)染效率，且所需RNAi濃度低，基因抑制效果更好，非特異性效應(yīng)極少
? 細(xì)胞毒性低、易于優(yōu)化，跨10倍轉(zhuǎn)染試劑濃度范圍均顯示了低細(xì)胞毒性
? 針對(duì)miRNA拮抗劑和模擬物具有出色的轉(zhuǎn)染效率
? 可用于多種細(xì)胞類型，是適合各種基因抑制實(shí)驗(yàn)的最通用方法
? 簡(jiǎn)單快速的實(shí)驗(yàn)方案，可獲得穩(wěn)定且可重復(fù)的結(jié)果

在多種細(xì)胞中可獲得很高的基因抑制效果
Lipofectamine? RNAiMAX轉(zhuǎn)染試劑可轉(zhuǎn)染各種類型的細(xì)胞(圖1)。在基因抑制應(yīng)用中，Lipofectamine? RNAiMAX轉(zhuǎn)染試劑的高效轉(zhuǎn)染提供了很高的基因抑制水平，足以獲得成功的結(jié)果。

簡(jiǎn)單、高通量的即用型轉(zhuǎn)染
只需將Lipofectamine? RNAiMAX轉(zhuǎn)染試劑與siRNA混合，加入細(xì)胞，在孵育所要求的時(shí)間后，檢測(cè)基因抑制效果。Lipofectamine? RNAiMAX轉(zhuǎn)染試劑使用簡(jiǎn)單、快速且具有很高的轉(zhuǎn)染效率，適用于高通量siRNA轉(zhuǎn)染。在上述應(yīng)用中，可輕松實(shí)現(xiàn)自動(dòng)化或機(jī)器人系統(tǒng)轉(zhuǎn)染條件的設(shè)定。

RNAiMAX轉(zhuǎn)染工作流程/ RNAiMAX轉(zhuǎn)染程序/ RNAiMAX轉(zhuǎn)染試劑的高效敲除

引用和文獻(xiàn)：

Mastermind-like Domain-containing 1 (MAMLD1 or CXorf6) Transactivates the Hes3 Promoter, Augments Testosterone Production, and Contains the SF1 Target Sequence.

• 作者:Fukami M, Wada Y, Okada M, Kato F, Katsumata N, Baba T, Morohashi KI, Laporte J, Kitagawa M, Ogata T,

• 期刊:J Biol Chem

• PubMed ID:18162467

Clathrin-dependent entry of severe acute respiratory syndrome coronavirus into target cells expressing ACE2 with the cytoplasmic tail deleted.

• 作者:Inoue Y, Tanaka N, Tanaka Y, Inoue S, Morita K, Zhuang M, Hattori T, Sugamura K,

• 期刊:J Virol

• PubMed ID:17522231

Post-transcriptional regulation of human pregnane X receptor by microRNA affects the expression of cytochrome P450 3A4.

• 作者:Takagi S, Nakajima M, Mohri T, Yokoi T,

• 期刊:J Biol Chem

• PubMed ID:18268015

Hypoxia-inducible factor 1alpha (HIF-1alpha)-mediated hypoxia increases BACE1 expression and beta-amyloid generation.

• 作者:Zhang X, Zhou K, Wang R, Cui J, Lipton SA, Liao FF, Xu H, Zhang YW,

• 期刊:J Biol Chem

• PubMed ID:17303576

Cycloheximide protects HepG2 cells from serum withdrawal-induced apoptosis by decreasing p53 and phosphorylated p53 levels.

• 作者:Bai J, Cederbaum AI,

• 期刊:J Pharmacol Exp Ther

• PubMed ID:16971506

Glomerulocystic kidney disease in mice with a targeted inactivation of Wwtr1.

• 作者:Hossain Z, Ali SM, Ko HL, Xu J, Ng CP, Guo K, Qi Z, Ponniah S, Hong W, Hunziker W,

• 期刊:Proc Natl Acad Sci U S A

• PubMed ID:17251353

An auto-regulatory loop between stress sensors INrf2 and Nrf2 controls their cellular abundance.

• 作者:Lee OH, Jain AK, Papusha V, Jaiswal AK,

• 期刊:J Biol Chem

• PubMed ID:17925401

Dual roles of myocardin-related transcription factors in epithelial mesenchymal transition via slug induction and actin remodeling.

• 作者:Morita T, Mayanagi T, Sobue K,

• 期刊:J Cell Biol

• PubMed ID:18056415

Diminished GATA4 protein levels contribute to hyperglycemia-induced cardiomyocyte injury.

• 作者:Kobayashi S, Mao K, Zheng H, Wang X, Patterson C, O'Connell TD, Liang Q,

• 期刊:J Biol Chem

• PubMed ID:17525155